Autoradiography in Chromatography

Autoradiography method is for the detection of radio active compounds. This is done by labeling the molecules in the test sample with a radioactive tracer. The developing of the chromatogram is as usual as in the previous methods. Then the chromatogram is dried or heated under sun or in an oven. An x-ray film is kept on top of the dried developed chromatogram. The compounds having radioactivity will be appeared as dark spots on the developed X-ray film. And these compounds are separated according to their radioactive ability.