Parts of High Performance Liquid Chromatography (HPLC)

HPLC instrument has four main systems and three other systems which help to proceed HPLC accurately. The four main systems include mobile phase reservoirs, injector system, column and detector. The other three are the pump, waste and the recorder. The four main systems will be discussing in detailed in next pages.

• Mobile phase reservoirs in HPLC
• Injector system in HPLC
• Column in HPLC
• Detector in HPLC

Pump mainly serves to move the mobile phase through the column. For this movement high pressure is required and it is given by the pump. The detector is connected to the waste and the recorder. Recorder will read the output given from the detector and it will produce peaks to indicate the detected compounds as relevant. Waste is to carry the unwanted outputs given from the detector.