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Observation Under Visible and UV Light in Chromatography


The colored compounds are easy to locate. And coloured compounds can observe using normal visible light. The colorless compounds are observed using UV light. The colourless compounds are located under UV light in two different wave lengths. Under 254 nm wave length the background of the filter paper will be light coloured and the spots will appear as dark spots. Fewer than 350 nm wave lengths the background of the filter paper will be dark and different coloured spots will be appeared.   


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