Paper Chromatography
The
mode of separation of paper chromatography is the partition and the basis for
the separation is solubility. As in other chromatographic techniques this also
has a stationary phase as well as a mobile phase. Both phases are liquids.
Stationary phase is water that is tightly bound to the paper. Filter papers are
used for this purpose and most common filter paper used is Whatman filter
paper- 98-99% Alpha cellulose. The cellulose paper can well absorb water
molecules. Fiber of cellulose acts as the stationary phase. Mobile phase is a
solvent- solvent partially miscible in water. Paper chromatography is usually
used for separating amino acids and anions and also testing histamines and antibiotics.
Paper
chromatography will be discussed as in the following.
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