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Mobile Phase Reservoirs in High Performance Liquid Chromatography

Solvent Reservoir

Mobile phase reservoir will carry the mobile phase solution and through the pump it will be pumped into the column. This movement is according to the gravitational force. The mobile phase reservoir is a solvent with different polarities such as water, methanol, and acetonitrile. 

These solvents should be highly pure. For an example, water should be used as de-ionized water. Hygienic conditions are facilitated with the use of 0.45 ┬Ám filter. The solvent is filtered through this filter mesh to remove all particulate matter. This is termed as Millipore filtering. Degas is done to minimize errors which occur when compounds interact with several gases. If degas or the removal of all the gases, is not done correctly then it may spoil the column.


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