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Spot Location in Chromatography

In chromatography after the development the main process is the spot identification. It is also termed as spot location. This is highly essential in order to verify the compounds present in the mixture. This process becomes complicated when identifying a complex compound. If the compounds are coloured the process becomes quite easy. But always this is not the case! Sometimes nothing is visible in the developed chromatogram. However we scientists need to clarify those in order to solve the problem further. Hence now days there are several techniques available to locate these spots. And with the improvement of the technology these have become very easy and also quick. Following are some of the methods of locating spots,

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