Spot Location in Chromatography
In chromatography after the development the
main process is the spot identification. It is also termed as spot location.
This is highly essential in order to verify the compounds present in the
mixture. This process becomes complicated when identifying a complex compound.
If the compounds are coloured the process becomes quite easy. But always this
is not the case! Sometimes nothing is visible in the developed chromatogram.
However we scientists need to clarify those in order to solve the problem
further. Hence now days there are several techniques available to locate these
spots. And with the improvement of the technology these have become very easy
and also quick. Following are some of the methods of locating spots,
- Observationunder visible light and UV light in chromatography
- Autoradiographyin chromatography
- Useof chemical reagents in chromatography
Comments
Post a Comment