High Performance Liquid Chromatography (HPLC)

High performance liquid chromatography is another method used for the compound separation. It is also termed as high pressure liquid chromatography. Unlike other chromatographic techniques this method is somewhat different due to its mobile phase’s unique character. Unlike other procedures the mobile phase of this HPLC moves due to pressure. In other words we can say mobile phase is moving in correlation with the gravitational force. In HPLC we will be focusing on the following topics.

• What is HPLC?
• Parts of HPLC
• Mobile phase reservoirs in HPLC
• Injector system in HPLC
• Column in HPLC
• Detector in HPLC
• Different physical principals for detecting compounds in HPLC
• Identification and quantification in HPLC