Like in other techniques, in HPLC the test sample is loaded into the stationary phase. But for this application HPLC has an injector system. The injector system loads the test sample into the stationary phase which is a column. Injector system contains an injector port, the place where the sample is injected.
In modernized equipments the injector port is auto sealed section which gradually seals itself after the sample is injected. This injector port is made from a slightly thick flexible material. Auto sealed injector ports should be changed with time by the user, because with time its auto sealing ability reduces. The sample is usually injected using a micro liter syringe.
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