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Quantification of Compounds in Paper Chromatography

The separated compounds on the chromatogram are first identified. Then the identified compounds are quantified according to the requirement. Quantification can be a comparative study among the separated compounds or it can be an accurate reading by a well performed instrument. Measuring the intensity of the colour under UV light is a comparative study to a certain extent. Another quantifying technique is done by cutting the chromatogram fragment where the separated compound of our interest is present. Then carrying out a chemical analysis of that piece of fragment. Measuring the radio activity using the Geiger Muller counter is a method used to quantify compounds in a highly accurate manner.

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