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Paper Chromatography Ascending Method

In ascending technique the chromatogram is attached in a way that the spot is touched with the solvent where the solvent is at the bottom. The development of the chromatogram or the separation of the spot is against the gravity. This is why this is termed as ascending technique. There is a paper support on the top of this tank. The mobile phase (solvent) is at the bottom of the tank. The filter paper is attached to the tank by the paper support and filter paper will touch the solvent. But the spot should not touch the solvent. The mobile phase will gradually rise up wards and carry the spot substances. The most polar substance will be at the bottom with respect to the tank where as the least polar will be on the top end of the tank. Ascending technique is relatively a slow process.

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