Special Chromatography Techniques

1. Reversed phase chromatography

A type of liquid chromatography. Mobile phase is more polar than the stationary phase (Not like other chromatography techniques)

2. Pyrolysis gas chromatography mass spectrometry

       -    Resulting simple molecules are subjected to a gas chromatography and then to a mass spectrometry.

       -    Test sample is heated to decompose into smaller molecules.

       -    Separated by GC.

       -    Quantified by mass spectroscopy

Pyrolysis:    Thermal decomposition of material in a vacuum material – quick heating 600- 1000 C

3. Countercurrent chromatography

-          A type of liquid – liquid chromatography

-          Physical principle is solubility.

-          Samples separate due to partition coefficient

-          Types of CCC – HPCCC (High performance CCC) , HSCCC(High Speed CCC)

-          Column rotates during separation.

4. Chiral chromatograph

-          Separates stereoisomer (Same formula with different arrangements)

-          A type of HPLC

-          Either mobile or stationary phase has to be Chiral.

-          Widely used in Pharmaceutical steroids.